Objectives: • To perform PCR amplification of specific target sequence from template DNA. • To analyzed the amplified product by Agarose gel electrophoresis.
Principle: PCR-polymerase chain reaction is an in vitro method of enzymatic synthesis of specific DNA sequence, developed by Kary Mullis in 1983. It is a very simple technique for characterizing, analyzing & synthesizing any specific DNA or RNA from any source.
Materials provided: (for 10 reactions) The list bellow provides the information about the materials supplied in the kit.
Materials Quantity Storage Condition Taq DNA polymerase 10 µl -20°C 10X Taq Buffer 60 µl -20°C Template DNA 10 µl -20°C Forward Primer 10 µl -20°C Reverse Primer 10 µl -20°C Nuclease free water 1 ml -20°C 1Kbp DNA ladder 50 µl -20°C dNTP mix 10 µl -20°C 6 X DNA Loading Dye 100 µl -20°C Agarose 1 gm RT 50 X TAE 15 ml 4°C PCR Tubes 10 Nos. RT Ethidium bromide (EtBr) 50 ul RT