Anti-Myc Antibody

Catalogue No. Quantity/Specifications Enquiry
Cat # BB-AB0045 100 µl (100 µg) Contact Us

Description

Product type: Primary Antibody
Clonality:       Polyclonal
Source:          Rabbit
Immunogen: Synthetic CEQKLISEEDL peptide conjugated to KLH.
Description: The Myc peptide tag is residues 410-419 of human c-Myc (EQKLISEEDL). Proteins of interest are generally tagged with Myc peptide for easy checking of their expression in recombinant strains. Anti-Myc antibody is the tool for detection of Myc-tag proteins by different immunological techniques. This antibody is produced in rabbit by repeated injections of Immunogen.

Purification: Purified against peptide affinity chromatography.
Specificity: Myc-tag polyclonal antibody detects Myc-tag recombinant proteins expressed in eukaryotic and prokaryotic cells. This antibody recognizes both C-terminal and N-terminal tags.
Recommended Dilutions:
Western Blotting  1:2,000
ELISA                     1:1,000
Storage buffer: Tris Buffer pH 7.4, containing 1% BSA & 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).
*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.



Technology

Anti-Myc Antibody

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