Product type: Primary Antibody
Immunogen: Synthetic 6 X HIS peptide conjugated to KLH.
Description: Proteins of interest are generally tagged with His peptide for both an easy detection and simple purification by Ni2+-affinity chromatography. Anti-His antibody is the tool for detection of His-tag proteins by different immunological techniques.
This antibody is produced in rabbit by repeated injections of Immunogen.
Purification: Purified against peptide affinity chromatography.
Specificity: His-tag polyclonal antibody detects His-tag
recombinant proteins expressed in eukaryotic and prokaryotic cells. This antibody recognizes both C-terminal and N-terminal tags.
Western Blotting 1:2000
Storage buffer: Tris Buffer pH 7.4, containing 1% BSA & 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).
*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1 X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.
1. Sci Rep. 2019 May 15;9(1):7450
Determination and analysis of agonist and antagonist potential of naturally occurring flavonoids for estrogen receptor (ERα) by various parameters and molecular modelling approach.
Puranik NV1,2, Srivastava P3,4, Bhatt G5, John Mary DJS5, Limaye AM5, Sivaraman J6.
2. Sci Rep. 2019 Jul 26;9(1):10882. doi: 10.1038/s41598-019-47244-y.
Newcastle disease virus mediated apoptosis and migration inhibition of human oral cancer cells: A probable role of β-catenin and matrix metalloproteinase-7.
Morla S1, Kumar A1, Kumar S2.
3. FASEB J. 2018 Oct 11:fj201801482R. doi: 10.1096/fj.201801482R. [Epub ahead of print]
HSF1 mediated TNF-α production during proteotoxic stress response pioneers proinflammatory signal in human cells.
Ali A1, Biswas A2, Pal M1.
4. PLoS One. 2017 Jan 18;12(1):e0168165.
Engagement of Components of DNA-Break Repair Complex and NFκB in Hsp70A1A Transcription Upregulation by Heat Shock.
Hazra J1, Mukherjee P1, Ali A1, Poddar S2, Pal M1
CHIP ASSAY done with BBL Anti-HIS-Ab
5. J Cell Sci. 2013 Apr 1;126(Pt 7):1650-8. doi: 10.1242/jcs.119859.
WISP3-IGF1 interaction regulates chondrocyte hypertrophy.
Repudi SR1, Patra M, Sen M.
6. J Immunol. 2014 May 1;192(9):4386-97. doi: 10.4049/jimmunol.1302817. Epub 2014 Apr 4.
Wnt5a-Rac1-NF-κB homeostatic circuitry sustains innate immune functions in macrophages.
Naskar D1, Maiti G, Chakraborty A, Roy A, Chattopadhyay D, Sen M.