Product type: Primary Antibody
Immunogen: Glutathione-S-transferase (GST) protein.
Description: GST is a 26 KDa protein encoded by the parasitic helminth Schistosom ajaponicum widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins, since it provides both an easily detectable tag and a simple purification process. Anti-GST antibody is the tool for detection of GST-tag proteins by different immunological techniques. This antibody is produced in rabbit by repeated injections of Immunogen.
Purification: Purified against protein affinity chromatography.
Specificity: Anti-GST polyclonal antibody detects GST-fussed recombinant proteins expressed in eukaryotic and prokaryotic cells.
Western Blotting 1:2000
Storage instructions: Store at -20°C (Recommended).
Storage buffer: PBS pH 7.3, containing 50% Glycerol without azide.
*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1 X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.
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