Product type: Primary Antibody
Immunogen: Synthetic DYKDDDDK peptide conjugated to KLH.
Description: Proteins of interest are generally tagged with FLAG peptide for easy checking of their expression in recombinant strains. Anti-FLAG antibody is the tool for detection of FLAG-tag proteins by different immunological techniques. This antibody is produced in rabbit by repeated injections of Immunogen.
Purification: Purified against peptide affinity chromatography.
Specificity: FLAG-tag polyclonal antibody detects FLAG-tag recombinant proteins expressed in eukaryotic and prokaryotic cells. This antibody recognizes both C-terminal and N-terminal tags.
Western Blotting 1:1000
Storage buffer: Tris Buffer pH 7.4, containing 1% BSA & 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).
*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1 X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.