Anti – γ Actin Antibody

Catalogue No. Quantity/Specifications Enquiry
Cat # BB-AB0025 100 µl (100 µg) Contact Us

Description

Product type: Primary Antibody
Clonality:        Polyclonal
Source:           Rabbit
Immunogen:  Recombinant mouse gamma actin protein
(residues 28-129) expressed in E. coli.

Description: Gamma actin is a ~42 KDa mammalian protein.
Anti-γ Actin antibody is primarily used to test purity of cytoplasmic cell fractionation and loading control. This antibody is produced in rabbit by repeated injections of Immunogen.

Purification: Purified against gamma actin protein (residues 28-129) affinity chromatography.

Specificity: This antibody detects gamma actin in eukaryotic cell extract and recombinant proteins expressed in prokaryotic cells.

Recommended Dilutions:
Western Blotting    1:2000
ELISA                       1:1000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).

*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubation and washings are in 1X TBS, 0.1% Tween-20.

Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.



Technology

Anti - γ Actin Antibody

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