Product type: Primary Antibody
Immunogen: Recombinant mouse gamma actin protein
(residues 28-129) expressed in E. coli.
Description: Gamma actin is a ~42 KDa mammalian protein.
Anti-γ Actin antibody is primarily used to test purity of cytoplasmic cell fractionation and loading control. This antibody is produced in rabbit by repeated injections of Immunogen.
Purification: Purified against gamma actin protein (residues 28-129) affinity chromatography.
Specificity: This antibody detects gamma actin in eukaryotic cell extract and recombinant proteins expressed in prokaryotic cells.
Western Blotting 1:2000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).
*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubation and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.
Anti - γ Actin AntibodyPDF DOWNLOAD