Product type: Primary Antibody
Immunogen: N-Terminal peptide of Human β Actin protein.
Description: Beta actin is a ~42 KDa mammalian protein. Anti-β actin antibody is primarily used to test purity of cytoplasmic cell fractionation and loading control. This antibody is produced in rabbit by repeated injections of Immunogen.
Purification: Purified against Beta actin peptide affinity chromatography.
Specificity: This antibody detects beta actin in eukaryotic cell extract.
Western Blotting 1:2,000-5,000
Storage Buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).
*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1 X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.
Anti β Actin AntibodyPDF DOWNLOAD