Product type: Primary Antibody
Immunogen: Recombinant N-terminal fragment of IKK γ protein (21 KDa) (residues 40-210) expressed in E. coli.
Description: The cytosolic NFκB/Rel transcription factors are in inactive state, complexed with the inhibitory IκB proteins. Phosphorylation-induced, proteasome-mediated degradation of IκB triggers their activation. The key regulatory step in this pathway involves activation of IκB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKγ and IKKβ serve as the catalytic subunits of the kinase. IKK serves as the regulatory subunit. Phosphorylation of serines 177 and 181 in the activation loop of IKKβ (176 and 180 in IKKα) causes conformational changes resulting in activation of IKK. Anti- IKKγ antibody can be used for IKK2/β kinase assay from immunoprecipitated (IP) cell extracts. This antibody is produced in rabbit by repeated injections of Immunogen.
Purification: Purified against IKKγ protein (residues 40-210) affinity chromatography.
Specificity: This antibody detects IKKγ protein (~48.2 KDa) in eukaryotic cell extracts and recombinant proteins expressed in prokaryotic cells.
Western Blotting 1:2,000
Storage buffer: Tris Buffer pH 7.4, containing 50% Glycerol without azide.
Storage instructions: Store at -20°C (Recommended).
*N. B. (Membrane blocking): One hr at RT in 5% (w/v) non-fat milk; (Primary Ab): Overnight at 4°C in 1% non-fat milk; (Secondary Ab): One hr at RT in 1% non-fat milk. All incubations and washings are in 1X TBS, 0.1% Tween-20.
Exp. Date: 18 months upon receiving at proper storage condition as mentioned in datasheet.
Anti-NEMO/IKKγ AntibodyPDF DOWNLOAD